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Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 min at room temperature

Cys 2 ) and cystine-free (Cys 2 -free) medium with vehicle (DMSO) or ferrostatin-1 (Fer-1, 1 M)

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Some people report no change in their drinking habits or desires